SpexFluorolog-3

Applications

• Fluorescence and absorption spectroscopy: measurement of the corrected fluorescence spectra at low concentrations and analysis of complex compounds exhibiting overlapping of the fluorescence and absorption spectra.

• Detection of substances and measurement of their concentrations: high sensitivity makes it possible to detect substances at very low concentrations and determine the trace concentrations of the biological fluorescence probes (e.g., subpicomolar concentration of fluorescein).

• Determination of the fluorescence quantum yield: the measurements of the excitation and integral fluorescence intensities allow determination of the fluorescence quantum yield.

• Fluorescence anisotropy measurements: the studies of the polarization characteristics of fluorescence enable one to determine the microviscosity, dynamics and phase transitions in biomembranes, etc.

• 3D spectroscopy: rapid recording (in a few minutes) of the fluorescence and absorption spectra makes it possible to reconstruct 3D excitation spectra.

• Measurements at a few excitation and fluorescence wavelengths: tuning of the excitation and detection monochromators allows application of the probes with several fluorescence or absorption maxima (pH-, calcium-, or magnesium-sensitive probes).

• Time-resolved measurements: measurements of the time evolution of the fluorescence polarization, anisotropy, absorption and fluorescence spectra.